Chlorambucil
/api/v1/drug/chlorambucilBoxed warning
LEUKERAN (chlorambucil) can severely suppress bone marrow function. Chlorambucil is a carcinogen in humans. Chlorambucil is probably mutagenic and teratogenic in humans. Chlorambucil produces human infertility (see WARNINGS and PRECAUTIONS).
Mechanism of action
Sourced from openFDAChlorambucil, an aromatic nitrogen mustard derivative, is an alkylating agent. Chlorambucil interferes with DNA replication and induces cellular apoptosis via the accumulation of cytosolic p53 and subsequent activation of Bax, an apoptosis promoter.
Indications
Sourced from openFDA- LEUKERAN (chlorambucil) is indicated in the treatment of chronic lymphatic (lymphocytic) leukemia, malignant lymphomas including lymphosarcoma, giant follicular lymphoma, and Hodgkin’s disease. It is not curative in any of these disorders but may produce clinically useful palliation.ICD-10: C85.90, C95.90
Contraindications
Sourced from openFDA- Chlorambucil should not be used in patients whose disease has demonstrated a prior resistance to the agent. Patients who have demonstrated hypersensitivity to chlorambucil should not be given the drug.contraindicated
Dosage & administration
Sourced from openFDAThe usual oral dosage is 0.1 to 0.2 mg/kg body weight daily for 3 to 6 weeks as required. This usually amounts to 4 to 10 mg per day for the average patient. The entire daily dose may be given at one time. These dosages are for initiation of therapy or for short courses of treatment. The dosage must be carefully adjusted according to the response of the patient and must be reduced as soon as there is an abrupt fall in the white blood cell count. Patients with Hodgkin’s disease usually require 0.2 mg/kg daily, whereas patients with other lymphomas or chronic lymphocytic leukemia usually require only 0.1 mg/kg daily. When lymphocytic infiltration of the bone marrow is present, or when the bone marrow is hypoplastic, the daily dose should not exceed 0.1 mg/kg (about 6 mg for the average patient). Alternate schedules for the treatment of chronic lymphocytic leukemia employing intermittent, biweekly, or once-monthly pulse doses of chlorambucil have been reported. Intermittent schedules of chlorambucil begin with an initial single dose of 0.4 mg/kg. Doses are generally increased by 0.1 mg/kg until control of lymphocytosis or toxicity is observed. Subsequent doses are modified to produce mild hematologic toxicity. It is felt that the response rate of chronic lymphocytic leukemia to the biweekly or once-monthly schedule of chlorambucil administration is similar or better to that previously reported with daily administration and that hematologic toxicity was less than or equal to that encountered in studies using daily chlorambucil.
Warnings & precautions
Sourced from openFDABecause of its carcinogenic properties, chlorambucil should not be given to patients with conditions other than chronic lymphatic leukemia or malignant lymphomas. Convulsions, infertility, leukemia, and secondary malignancies have been observed when chlorambucil was employed in the therapy of malignant and non-malignant diseases. There are many reports of acute leukemia arising in patients with both malignant and non-malignant diseases following chlorambucil treatment. In many instances, these patients also received other chemotherapeutic agents or some form of radiation therapy. The quantitation of the risk of chlorambucil-induction of leukemia or carcinoma in humans is not possible. Evaluation of published reports of leukemia developing in patients who have received chlorambucil (and other alkylating agents) suggests that the risk of leukemogenesis increases with both chronicity of treatment and large cumulative doses. However, it has proved impossible to define a cumulative dose below which there is no risk of the induction of secondary malignancy. The potential benefits from chlorambucil therapy must be weighed on an individual basis against the possible risk of the induction of a secondary malignancy. Chlorambucil has been shown to cause chromatid or chromosome damage in humans. Both reversible and permanent sterility have been observed in both sexes receiving chlorambucil. A high incidence of sterility has been documented when chlorambucil is administered to prepubertal and pubertal males. Prolonged or permanent azoospermia has also been observed in adult males.
Adverse reactions
Sourced from openFDATo report SUSPECTED ADVERSE REACTIONS, contact Waylis Therapeutics LLC Toll-Free at 1-888-514-4727 or FDA at 1-800-FDA-1088 or www.fda.gov/medwatch. Hematologic The most common side effect is bone marrow suppression, anemia, leukopenia, neutropenia, thrombocytopenia, or pancytopenia. Although bone marrow suppression frequently occurs, it is usually reversible if the chlorambucil is withdrawn early enough. However, irreversible bone marrow failure has been reported. Gastrointestinal Gastrointestinal disturbances such as nausea and vomiting, diarrhea, and oral ulceration occur infrequently. CNS Tremors, muscular twitching, myoclonia, confusion, agitation, ataxia, flaccid paresis, and hallucinations have been reported as rare adverse experiences to chlorambucil which resolve upon discontinuation of drug. Rare, focal and/or generalized seizures have been reported to occur in both children and adults at both therapeutic daily doses and pulse-dosing regimens, and in acute overdose (see PRECAUTIONS: General). Dermatologic Allergic reactions such as urticaria and angioneurotic edema have been reported following initial or subsequent dosing. Skin hypersensitivity (including rare reports of skin rash progressing to erythema multiforme, toxic epidermal necrolysis, and Stevens-Johnson syndrome) has been reported (see WARNINGS). Miscellaneous Other reported adverse reactions include: pulmonary fibrosis, hepatotoxicity and jaundice, drug fever, peripheral neuropathy, interstitial pneumonia, sterile cystitis, infertility, leukemia, and secondary malignancies (see WARNINGS).
Use in specific populations
Sourced from openFDAPregnancy Chlorambucil can cause fetal harm when administered to a pregnant woman. Unilateral renal agenesis has been observed in 2 offspring whose mothers received chlorambucil during the first trimester. Urogenital malformations, including absence of a kidney, were found in fetuses of rats given chlorambucil. There are no adequate and well-controlled studies in pregnant women. If this drug is used during pregnancy, or if the patient becomes pregnant while taking this drug, the patient should be apprised of the potential hazard to the fetus. Women of childbearing potential should be advised to avoid becoming pregnant.
Pharmacokinetics
Sourced from openFDA- Metabolism
- In a study of 12 patients given single oral doses of 0.2 mg/kg of LEUKERAN, the mean dose-adjusted (±SD) plasma chlorambucil C max was 492 ± 160 ng/mL, the AUC was 883 ± 329 ng.h/mL, the mean elimination half-life (t½) was 1.3 ± 0.5 hours, and the T max was 0.83 ± 0.53 hours. For the major metabolite, phenylacetic acid mustard (PAAM), the mean dose-adjusted (± SD) plasma C max was 306 ± 73 ng/mL, the AUC was 1204 ± 285 ng.h/mL, mean t½ was 1.8 ± 0.4 hours, and the T max was 1.9 ± 0.7 hours.
Overdosage
Sourced from openFDAReversible pancytopenia was the main finding of inadvertent overdoses of chlorambucil. Neurological toxicity ranging from agitated behavior and ataxia to multiple grand mal seizures has also occurred. As there is no known antidote, the blood picture should be closely monitored and general supportive measures should be instituted, together with appropriate blood transfusions, if necessary. Chlorambucil is not dialyzable. Oral LD 50 single doses in mice are 123 mg/kg. In rats, a single intraperitoneal dose of 12.5 mg/kg of chlorambucil produces typical nitrogen-mustard effects; these include atrophy of the intestinal mucous membrane and lymphoid tissues, severe lymphopenia becoming maximal in 4 days, anemia, and thrombocytopenia. After this dose, the animals begin to recover within 3 days and appear normal in about a week, although the bone marrow may not become completely normal for about 3 weeks. An intraperitoneal dose of 18.5 mg/kg kills about 50% of the rats with development of convulsions. As much as 50 mg/kg has been given orally to rats as a single dose, with recovery. Such a dose causes bradycardia, excessive salivation, hematuria, convulsions, and respiratory dysfunction.
Approval history
Sourced from openFDA- Mar 18, 1957NDANDA010669Waylis Therap
FAERS reports
- 1Neutropenia2747.0%
- 2Pneumonia2656.8%
- 3Off Label Use2616.7%
- 4Pyrexia2376.1%
- 5Drug Ineffective1965.0%
- 6Thrombocytopenia1914.9%
- 7Febrile Neutropenia1624.2%
- 8Anaemia1574.0%
- 9Disease Progression1544.0%
- 10Acute Myeloid Leukaemia1313.4%
- 11Dyspnoea1253.2%
- 12Infection1233.2%
- 13Death1153.0%
- 14Progressive Multifocal Leukoencephalopathy1112.9%
- 15Nausea1092.8%
Literature
Recent PubMed references pinned to Chlorambucil as a MeSH major topic. Citations link to pubmed.ncbi.nlm.nih.gov.
- Tromethamine‑Modified Chlorambucil Prodrug Nano-Micelles: Improved Colloidal Stability and Antitumor Efficacy.International journal of nanomedicine · 2026 · Li Z, Xu S, Jiang X, et al.PMID 42170006DOI 10.2147/IJN.S606369
- Investigation of cytotoxic, molecular and in silico effects of chlorambucil and tamoxifen on 2D/3D MDA-MB-231 and HeLa cancer cell models.Molecular biology reports · 2025 · Demirkaya DB, Arslan HS, Azarkan SY, et al.PMID 41335253DOI 10.1007/s11033-025-11286-5
- Cathepsin B-Activated Prodrug for Precision Tumor Theranostics.Journal of medicinal chemistry · 2025 · Zhai W, Li J, Zhao D, et al.PMID 41042236DOI 10.1021/acs.jmedchem.5c02241
- Novel therapeutic strategy targeting STMN1 using chlorambucil-conjugated pyrrole-imidazole polyamide in small cell lung cancer.International journal of cancer · 2026 · Yoshikawa R, Watanabe T, Ohtaki Y, et al.PMID 41025286DOI 10.1002/ijc.70179
- Unlocking the Therapeutic Synergy of Nitric Oxide (NO) and Chlorambucil (Cbl) via Photoresponsive Sequential Delivery in a Triple-Negative Breast Cancer 3D Spheroidal Platform with Transcriptomic Insights.Journal of medicinal chemistry · 2025 · Sarampally V, Poddar S, Trivedi P, et al.PMID 40931690DOI 10.1021/acs.jmedchem.5c01995
- In silico design and evaluation of a hydrophobic-hydrophilic combinatorial drug systems for targeted breast cancer therapy.Biochemical and biophysical research communications · 2025 · Muthumanickam S, Manikandan M, Pandi B, et al.PMID 40848547DOI 10.1016/j.bbrc.2025.152499
- Self-immolative PEGylated dendritic drug conjugates for cancer therapy with enhanced cellular uptake.Chemical communications (Cambridge, England) · 2025 · Xia Z, Zhang Y, Zuo X, et al.PMID 40525546DOI 10.1039/d5cc02776d
- Exploring the Phototherapeutic Applications of Mitochondria-Targeted COUPY Photocages of Antitumor Drugs.Journal of medicinal chemistry · 2025 · López-Corrales M, Izquierdo-García E, Bosch M, et al.PMID 40293412DOI 10.1021/acs.jmedchem.5c00550
Clinical trials
The 10 most recently updated of 65 ClinicalTrials.gov registrations naming Chlorambucil as an intervention. Registration is not evidence of efficacy or safety — reference crosswalk only.
- A Study of the Combination of Ibrutinib Plus Venetoclax Versus Chlorambucil Plus Obinutuzumab for the First-line Treatment of Participants With Chronic Lymphocytic Leukemia (CLL)/Small Lymphocytic Lymphoma (SLL)Active not recruiting · Phase 3 · Interventional · 211 enrolled · Janssen Research & Development, LLCNCT03462719updated 2026-06-08
- Study of Acalabrutinib Versus Chlorambucil Plus Rituximab in Adult Subjects With Previously Untreated Chronic Lymphocytic LeukemiaActive not recruiting · Phase 3 · Interventional · 155 enrolled · AstraZenecaNCT04075292updated 2026-03-05
- Phase II Study of Chlorambucil and Subcutaneous Rituximab in Patients With Extranodal MALT LymphomaActive not recruiting · Phase 2 · Interventional · 112 enrolled · International Extranodal Lymphoma Study Group (IELSG)NCT01808599updated 2026-01-15
- Comparison of the Treatments of Obinutuzumab + Venetoclax Versus Obinutuzumab + Chlorambucil in Patients With Chronic Lymphocytic LeukemiaCompleted · Phase 3 · Interventional · 445 enrolled · Hoffmann-La RocheNCT02242942updated 2025-11-06
- Acalabrutinib, Obinutuzumab and Chlorambucil in Treatment naïve CLLActive not recruiting · Phase 3 · Interventional · 535 enrolled · Acerta Pharma BVNCT02475681updated 2025-08-27
- Ublituximab + TGR-1202 Compared to Obinutuzumab + Chlorambucil in Participants With Untreated and Previously Treated Chronic Lymphocytic LeukemiaTerminated · Phase 3 · Interventional · 603 enrolled · TG Therapeutics, Inc.NCT02612311updated 2024-12-13
- Open-label Extension Study in Patients 65 Years or Older With Chronic Lymphocytic Leukemia or Small Lymphocytic LymphomaCompleted · Phase 3 · Interventional · 269 enrolled · Pharmacyclics LLC.NCT01724346updated 2024-09-19
- A Global Study of Lisaftoclax (APG-2575) Combined With Acalabrutinib Versus Immunochemotherapy for Newly Diagnosed CLL/SLL.Recruiting · Phase 3 · Interventional · 344 enrolled · Ascentage Pharma Group Inc.NCT06319456updated 2024-05-29
- A Single Arm Study of Acalabrutinib Conbimed With Obinutuzumab in Chinese Patients With Previously Untreated CLLRecruiting · Interventional · 89 enrolled · The First Affiliated Hospital with Nanjing Medical UniversityNCT05950997updated 2024-05-09
- Chlorambucil in Metastatic PDAC Patients Bearing a Germ Line DNA Defects Repair Mutations (SALE Trial)Unknown · Phase 2 · Interventional · 20 enrolled · Michele ReniNCT04692740updated 2023-09-21
Frequently asked questions
- How does Chlorambucil work?
- Chlorambucil, an aromatic nitrogen mustard derivative, is an alkylating agent. Chlorambucil interferes with DNA replication and induces cellular apoptosis via the accumulation of cytosolic p53 and subsequent activation of Bax, an apoptosis promoter.
- What is Chlorambucil used for?
- According to FDA labeling, Chlorambucil carries indications including: LEUKERAN (chlorambucil) is indicated in the treatment of chronic lymphatic (lymphocytic) leukemia, malignant lymphomas including lymphosarcoma, giant follicular lymphoma, and Hodgkin’s disease. It is not curative in any of these disorders but may produce clinically useful palliation.. This is a reference summary of labeled uses, not medical advice or a treatment recommendation.
- What class of drug is Chlorambucil?
- Chlorambucil is classified as Nitrogen mustard analogues, Alkylating Drug, Alkylating Activity, Decreased DNA Integrity, Decreased DNA Replication.
- What are the brand names for Chlorambucil?
- Chlorambucil is marketed under brand names including Leukeran.
- What are the contraindications for Chlorambucil?
- Chlorambucil labeling lists contraindications including: Chlorambucil should not be used in patients whose disease has demonstrated a prior resistance to the agent. Patients who have demonstrated hypersensitivity to chlorambucil should not be given the drug.. Always consult the full prescribing information and a clinician.
chlorambucil is illustrative MVP content compiled from public sources. pharmacopeia is for educational and informational use only and is not a substitute for professional medical advice.