Levoleucovorin
/api/v1/drug/levoleucovorinMechanism of action
Sourced from openFDAHigh-Dose Methotrexate Therapy Levoleucovorin is the pharmacologically active isomer of 5-formyl tetrahydrofolic acid. Levoleucovorin does not require reduction by the enzyme dihydrofolate reductase in order to participate in reactions utilizing folates as a source of “one-carbon” moieties.
Indications
Sourced from openFDA- Levoleucovorin Injection is indicated for: rescue after high-dose methotrexate therapy in adult and pediatric patients with osteosarcoma. diminishing the toxicity associated with overdosage of folic acid antagonists or impaired methotrexate elimination in adult and pediatric patients.
Contraindications
Sourced from openFDA- Levoleucovorin is contraindicated in patients who have had severe hypersensitivity to leucovorin products, folic acid or folinic acid [see Adverse Reactions ( 6.2 )] . Patients who have had severe hypersensitivity reactions to leucovorin products, folic acid or folinic acid.contraindicated
Dosage & administration
Sourced from openFDAFor intravenous administration only. Do not administer intrathecally. ( 2.1 ) Rescue After High-Dose Methotrexate Therapy Rescue recommendations are based on methotrexate dose of 12 grams/m 2 administered by intravenous infusion over 4 hours. Initiate rescue at a dose of 7.5 mg (approximately 5 mg/m 2 ) every 6 hours, 24 hours after the beginning of methotrexate infusion. ( 2.3 ) Continue until the methotrexate level is below 5 x 10 -8 M (0.05 micromolar). Adjust dose if necessary based on methotrexate elimination; refer to Full Prescribing Information. ( 2.3 ) Overdosage of Folic Acid Antagonists or Impaired Methotrexate Elimination Start as soon as possible after methotrexate overdosage or within 24 hours of delayed methotrexate elimination. ( 2.4 ) Administer levoleucovorin injection 7.5 mg (approximately 5 mg/m 2 ) intravenously every 6 hours until methotrexate level is less than 5 x 10 -8 M (0.05 micromolar). ( 2.4 ) Metastatic Colorectal Cancer in Combination with Fluorouracil The following regimens have been used for the treatment of colorectal cancer: Levoleucovorin injection 100 mg/m 2 by intravenous injection over a minimum of 3 minutes, followed by fluorouracil 370 mg/m 2 once daily for 5 consecutive days. ( 2.5 ) Levoleucovorin injection 10 mg/m 2 by intravenous injection followed by fluorouracil 425 mg/m 2 once daily for 5 consecutive days. ( 2.5 ) Administer fluorouracil and levoleucovorin injection separately to avoid the formation of precipitate.
Warnings & precautions
Sourced from openFDAHypercalcemia: Due to calcium content, inject no more than 16 mL (160 mg) of levoleucovorin solution intravenously per minute. ( 5.1 ) Increased Gastrointestinal Toxicities with Fluorouracil : Do not initiate or continue therapy with levoleucovorin and fluorouracil in patients with symptoms of gastrointestinal toxicity until symptoms have resolved. Monitor patients with diarrhea until it has resolved as rapid deterioration leading to death can occur. ( 5.2 , 7 ) Drug Interaction with Trimethoprim-Sulfamethoxazole : Increased rates of treatment failure and morbidity with concomitant use of d,l- leucovorin with trimethoprim-sulfamethoxazole for Pneumocystis jiroveci pneumonia in patients with HIV. ( 5.3 ) 5.1 Hypercalcemia Because of the calcium content of the levoleucovorin solution, inject no more than 16 mL (160 mg of levoleucovorin) intravenously per minute. 5.2 Increased Gastrointestinal Toxicities with Fluorouracil Leucovorin products increase the toxicities of fluorouracil [see Drug Interactions ( 7 )] . Gastrointestinal toxicities, including stomatitis and diarrhea, occur more commonly and may be of greater severity and of prolonged duration. Deaths from severe enterocolitis, diarrhea, and dehydration have occurred in elderly patients receiving weekly d,l- leucovorin and fluorouracil. Monitor patients for gastrointestinal toxicities. Do not initiate or continue therapy with levoleucovorin and fluorouracil in patients with symptoms of gastrointestinal toxicity until those symptoms have resolved.
Adverse reactions
Sourced from openFDAThe following clinically significant adverse reactions are described elsewhere in the labeling: Hypercalcemia [see Warnings and Precautions ( 5.1 )] Increased gastrointestinal toxicities with fluorouracil [see Warnings and Precautions ( 5.2 )] The most common adverse reactions (≥20%) in patients receiving high-dose methotrexate therapy with levoleucovorin rescue are stomatitis and vomiting. ( 6.1 ) The most common adverse reactions (>50%) in patients receiving levoleucovorin in combination with fluorouracil for metastatic colorectal cancer are stomatitis, diarrhea, and nausea. ( 6.1 ) To report SUSPECTED ADVERSE REACTIONS, contact Meitheal Pharmaceuticals Inc. at 1-844-824-8426 or FDA at 1-800-FDA-1088 or www.fda.gov/medwatch . 6.1 Clinical Trials Experience Because clinical trials are conducted under widely varying conditions, adverse reaction rates observed in the clinical trials of a drug cannot be directly compared to rates in the clinical trials of another drug and may not reflect the rates observed in practice. High-Dose Methotrexate Therapy Table 2 presents the frequency of adverse reactions which occurred during the administration of 58 courses of high-dose methotrexate 12 grams/m 2 followed by levoleucovorin rescue for osteosarcoma in 16 patients aged 6 to 21 years. Most patients received levoleucovorin 7.5 mg every 6 hours for 60 hours or longer, beginning 24 hours after completion of methotrexate administration.
Use in specific populations
Sourced from openFDA8.1 Pregnancy Risk Summary There are limited data with levoleucovorin use in pregnant women. Animal reproduction studies have not been conducted with levoleucovorin. Levoleucovorin is administered in combination with methotrexate or fluorouracil, which can cause embryo-fetal harm. Refer to methotrexate and fluorouracil prescribing information for additional information. In the U.S. general population, the estimated background risk of major birth defects and miscarriage in clinically recognized pregnancies is 2% to 4% and 15% to 20%, respectively. 8.2 Lactation Risk Summary There are no data on the presence of levoleucovorin in human milk or its effects on the breastfed infant or on milk production. Levoleucovorin is administered in combination with methotrexate or fluorouracil. Refer to methotrexate and fluorouracil prescribing information for additional information. 8.4 Pediatric Use The safety and effectiveness of levoleucovorin have been established in pediatric patients for rescue after high-dose methotrexate therapy in osteosarcoma and diminishing the toxicity associated with overdosage of folic acid antagonists or impaired methotrexate elimination. Use of levoleucovorin in pediatric patients is supported by open-label clinical trial data in 16 pediatric patients 6 years of age and older, with additional supporting evidence from literature [see Clinical Studies ( 14.1 )].
Pharmacokinetics
Sourced from openFDA- Metabolism
- The pharmacokinetics of levoleucovorin after intravenous administration of a 15 mg dose was studied in healthy subjects. The mean maximum serum total tetrahydrofolate (total-THF) concentrations was 1722 ng/mL (CV 39%) and the mean maximum serum (6S)-5-methyl-5,6,7,8-tetrahydrofolate concentrations was 275 ng/mL (CV 18%) observed around 0.9 hours post injection.
Overdosage
Sourced from openFDARecommended Dosage for Overdosage of Folic Acid Antagonists or Impaired Methotrexate Elimination Start levoleucovorin injection as soon as possible after an overdosage of methotrexate or within 24 hours of methotrexate administration when methotrexate elimination is impaired. As the time interval between methotrexate administration and levoleucovorin injection increases, the effectiveness of levoleucovorin injection to diminish methotrexate toxicity may decrease. Administer levoleucovorin injection 7.5 mg (approximately 5 mg/m 2 ) by intravenous infusion every 6 hours until the serum methotrexate level is less than 5 x 10 -8 M (0.05 micromolar). Monitor serum creatinine and methotrexate levels at least every 24 hours.
Approval history
Sourced from openFDA- Jun 16, 2016ANDAANDA206263Hikma
- Sep 8, 2017ANDAANDA207548Amneal
- Sep 14, 2018ANDAANDA210892Gland
- Oct 19, 2018NDANDA211226Acrotech Biopharma
- Aug 16, 2019ANDAANDA211002Meitheal
- Aug 22, 2019ANDAANDA211003Meitheal
- May 22, 2023ANDAANDA217314Hainan Poly Pharm
FAERS reports
- 1Off Label Use2747.7%
- 2Nausea2727.6%
- 3Neutropenia2456.9%
- 4Diarrhoea2406.7%
- 5Decreased Appetite1795.0%
- 6Vomiting1604.5%
- 7Neuropathy Peripheral1494.2%
- 8Malignant Neoplasm Progression1484.1%
- 9Pyrexia1353.8%
- 10Asthenia1323.7%
- 11Neutrophil Count Decreased1293.6%
- 12Myelosuppression1263.5%
- 13Thrombocytopenia1263.5%
- 14Anaemia1183.3%
- 15Interstitial Lung Disease1052.9%
Literature
Recent PubMed references pinned to Levoleucovorin as a MeSH major topic. Citations link to pubmed.ncbi.nlm.nih.gov.
- Three-Period Bioequivalence Study of Sodium Levofolinate Injection With Calcium Levofolinate for Injection and Sodium Folinate for Injection in Healthy Chinese Subjects.Clinical pharmacology in drug development · 2023 · Qiu B, Liu G, Wang C, et al.PMID 36808267DOI 10.1002/cpdd.1223
- Stability of calcium levofolinate reconstituted in syringes and diluted in NaCl 0.9% and glucose 5% polyolefin/polyamide infusion bags.Journal of oncology pharmacy practice : official publication of the International Society of Oncology Pharmacy Practitioners · 2021 · Sanogo S, Silimbani P, Gaggeri R, et al.PMID 32299315DOI 10.1177/1078155220918025
- Levofolene modulates apoptosis induced by 5-fluorouracil through autophagy inhibition: clinical and occupational implications.International journal of oncology · 2015 · Lamberti M, Porto S, Zappavigna S, et al.PMID 25709090DOI 10.3892/ijo.2015.2904
- A pharmacokinetic and pharmacodynamic investigation of Modufolin® compared to Isovorin® after single dose intravenous administration to patients with colon cancer: a randomized study.Cancer chemotherapy and pharmacology · 2015 · Wettergren Y, Taflin H, Odin E, et al.PMID 25342290DOI 10.1007/s00280-014-2611-9
- Levoleucovorin as replacement for leucovorin in cancer treatment.The Annals of pharmacotherapy · 2012 · Chuang VT, Suno MPMID 23032661DOI 10.1345/aph.1Q677
- Evaluation of efficacy and safety of generic levofolinate in patients who received colorectal cancer chemotherapy.Medical oncology (Northwood, London, England) · 2011 · Fujii H, Iihara H, Yasuda K, et al.PMID 20354823DOI 10.1007/s12032-010-9487-2
Clinical trials
The 10 most recently updated of 1,279 ClinicalTrials.gov registrations naming Levoleucovorin as an intervention. Registration is not evidence of efficacy or safety — reference crosswalk only.
- Substudy 06E: Umbrella Study of Combination Therapies in Esophageal Cancer (MK-3475-06E/KEYMAKER-U06)Recruiting · Phase 1 · Phase 2 · Interventional · 298 enrolled · Merck Sharp & Dohme LLCNCT06780111updated 2026-06-12
- Targeted Therapy Directed by Genetic Testing in Treating Patients With Locally Advanced or Advanced Solid Tumors, The ComboMATCH Screening TrialRecruiting · Phase 2 · Interventional · 2,900 enrolled · National Cancer Institute (NCI)NCT05564377updated 2026-06-12
- A Study to Assess Intravenous (IV) Telisotuzumab Adizutecan in Combination With Fluorouracil, Folinic Acid, and Oxaliplatin (FOLFOX) Compared to Standard of Care in Adult Participants With First-Line Metastatic Pancreatic Ductal AdenocarcinomaRecruiting · Phase 2 · Phase 3 · Interventional · 900 enrolled · AbbVieNCT07490301updated 2026-06-12
- A Study to Evaluate the Adverse Events, and Efficacy of Intravenous (IV) of Telisotuzumab Adizutecan in Combination With IV Oxaliplatin, Fluorouracil, Folinic Acid/Leucovorin, Bevacizumab, Panitumumab in Adult Participants With Metastatic Colorectal CancerRecruiting · Phase 2 · Interventional · 390 enrolled · AbbVieNCT06820463updated 2026-06-12
- A Study of Fruquintinib Plus FOLFIRI as Second-Line Treatment for Participants With Metastatic Colorectal Cancer (FRUITFUL)Recruiting · Phase 2 · Interventional · 60 enrolled · SCRI Development Innovations, LLCNCT07011576updated 2026-06-12
- Personalize (Signature Driven) Neoadjuvant Chemotherapy Trial for Patients With Resectable Borderline Pancreatic Ductal Adenocarcinoma.Not yet recruiting · Phase 2 · Interventional · 110 enrolled · Federation Francophone de Cancerologie DigestiveNCT07616362updated 2026-06-11
- A Study of Encorafenib Plus Cetuximab With or Without Chemotherapy in People With Previously Untreated Metastatic Colorectal CancerActive not recruiting · Phase 3 · Interventional · 841 enrolled · PfizerNCT04607421updated 2026-06-11
- Chemotherapy Followed by Pelvic Reirradiation Versus Chemotherapy Alone as Pre-operative Treatment for Locally Recurrent Rectal CancerActive not recruiting · Phase 3 · Interventional · 58 enrolled · University Hospital, BordeauxNCT03879109updated 2026-06-11
- Testing the Addition of the Anti-cancer Drug Venetoclax and/or the Anti-cancer Immunotherapy Blinatumomab to the Usual Chemotherapy Treatment for Infants With Newly Diagnosed KMT2A-rearranged or KMT2A-non-rearranged LeukemiaRecruiting · Phase 2 · Interventional · 153 enrolled · National Cancer Institute (NCI)NCT06317662updated 2026-06-11
- A Placebo-controlled Trial of Folinic Acid in Children With ASDNot yet recruiting · Phase 2 · Interventional · 150 enrolled · Prof. Adi AranNCT07642661updated 2026-06-11
Frequently asked questions
- How does Levoleucovorin work?
- High-Dose Methotrexate Therapy Levoleucovorin is the pharmacologically active isomer of 5-formyl tetrahydrofolic acid. Levoleucovorin does not require reduction by the enzyme dihydrofolate reductase in order to participate in reactions utilizing folates as a source of “one-carbon” moieties.
- What is Levoleucovorin used for?
- According to FDA labeling, Levoleucovorin carries indications including: Levoleucovorin Injection is indicated for: rescue after high-dose methotrexate therapy in adult and pediatric patients with osteosarcoma. diminishing the toxicity associated with overdosage of folic acid antagonists or impaired methotrexate elimination in adult and pediatric patients.. This is a reference summary of labeled uses, not medical advice or a treatment recommendation.
- What class of drug is Levoleucovorin?
- Levoleucovorin is classified as Folate Analog.
- What are the brand names for Levoleucovorin?
- Levoleucovorin is marketed under brand names including Fusilev, Khapzory.
- What are the contraindications for Levoleucovorin?
- Levoleucovorin labeling lists contraindications including: Levoleucovorin is contraindicated in patients who have had severe hypersensitivity to leucovorin products, folic acid or folinic acid [see Adverse Reactions ( 6.2 )] . Patients who have had severe hypersensitivity reactions to leucovorin products, folic acid or folinic acid.. Always consult the full prescribing information and a clinician.
levoleucovorin is illustrative MVP content compiled from public sources. pharmacopeia is for educational and informational use only and is not a substitute for professional medical advice.